Fig. 5

RGMa inhibition abolishes TGFβ1-induced phosphorylation of Smad2/3. a, b Western blot analysis for phosphorylation and expression of Smad2 a and Smad3 b in primary astrocyte cultures infected with rAd-shRGMa or rAd-HK 3 days before exposure to TGFβ1 (10 ng/ml for 3 days; n = 3). NS, no significance. Data in bar graphs are means ± SEM (a, b); one-way ANOVA with Bonferroni post hoc test, **p < 0.01