Fig. 5
From: Polycomb protein family member CBX7 regulates intrinsic axon growth and regeneration
Overexpression of Cbx7 inhibits mammalian axon regeneration in DRG neurons in vivo. a Representative images of axon regeneration of sciatic nerves. After transfection of GFP and Cbx7-OE plasmid into the DRGs, mice were subjected to a sciatic nerve crush procedure. Using the whole-mount nerve segment, the lengths of all identifiable regenerating axons were measured from the crush site (marked by epineural suture, red line) to the distal axon tips. b CBX7 high expression is shown after electroporation of Cbx7-OE plasmid into adult DRG neurons by western blotting. c Quantification of axon regeneration length (GFP, n = 6; Cbx7-OE, n = 6) are shown (n = 284 for control; n = 268 for Cbx 7-OE). Scale bar, 1 mm. d In vivo regulation index of axon lengths of all individual axons measured (n = 284 for control; n = 268 for Cbx7-OE). e Cumulative distribution of the lengths of all individual axons measured (n = 284 for control; n = 268 for Cbx 7-OE)
