Fig. 6

CBX7 Targets SOX11 and GATA4 for controlling axon regeneration in adult DRG neurons. a Relative gene expression levels after CBX7 knockdown in cultured DRG sensory neurons (n = 4, *p < 0.05, **p < 0.01). b–e The mRNA levels of Wnt1, Wnt10b, Sox11, and Gata4 are significantly increased in sensory neurons in vivo 7 days after sciatic nerve injury. (b, n = 9; c, n = 6; d, n = 7; e, n = 4; f, n = 3; *p < 0.05, **p < 0.01, ***p < 0.0001). f The enrichment of CBX protein at genomic sequence upstream of the target gene locus in DRG tissues, as assessed by CBX7-specific chromatin immunoprecipitation (ChIP). ChIP with IgG antibody in DRG tissues was used as negative controls. Relative enrichment of CBX7 to each target gene in DRG tissues was calculated relative to IgG-only nonspecific control in the same group (n = 4, *p < 0.05, **p < 0.01). Quantities were calculated from an input DNA-generated standard curve. Two-way ANOVA, Bonferroni post test. g The enrichment of CBX protein at genomic sequence upstream of the target gene locus in regenerated cultured DRG neurons, as assessed by CBX7-specific ChIP. (n = 3, *p < 0.05, **p < 0.01). Quantities were calculated from an input DNA-generated standard curve. Two-way ANOVA, Bonferroni post test. h Gata4 mRNA (n = 3; ** P < 0.01) level was increased in adult DRGs 3 days after in vivo electroporation of Cbx 7 siRNA. i Sox11 mRNA (n = 3; ** P < 0.01) level was increased in adult DRGs 3 days after in vivo electroporation of Cbx7 siRNA