Fig. 7
From: Polycomb protein family member CBX7 regulates intrinsic axon growth and regeneration
Downregulation of SOX11 restores the axon regeneration phenotype exhibited by CBX7 knockdown in adult DRG neurons. a Comparison of Sox11 expression level in cultured DRG sensory neurons between DIV0 and DIV3. Sox11 expression level is much higher at DIV3 than those at DIV0 (n = 6, **p < 0.01). b Sox11 mRNA level was tested after 3 days of siSOX11 electroporation into DRG sensory neurons (n = 3, *p < 0.05) and the mRNA level of Sox11 quantified using qRT-PCR and normalized to the expression of the GAPDH. c Adult DRG neurons were transfected with GFP plus siRNA ctrl and siRNAs against SOX11 (siSOX11) as indicated. At 3 days after transfection, neurons were fixed and immunostained with anti-GFP and anti-TuJ1 antibodies. Representative images of neurons transfected with siRNAs and GFP are shown. d Quantification of axon length of adult DRG neurons transfected with GFP or siSox11 (n = 148 for control, n = 206 for siSOX11) (n = 3, *P < 0.05). Scale bar, 100 µm. e Representative images of cultured adult sensory neurons showing that knocking down SOX11 (siSox11) restored regenerative axon growth phenotype by knocking down CBX7 (siCBX7). At Day 4 after transfection, neurons were fixed and immunostained with anti-GFP and anti-TuJ1 antibodies. Scale bar, 100 µm. f Quantification of axon length of adult DRG neurons transfected with GFP, siCBX7, siSOX11, and siCBX7 plus siSOX11 (n = 271 for control, n = 205 for siCBX7, n = 204 for siSOX11, and n = 177 for siCBX7 + siSOX11) (n = 5, *p < 0.05)
