Fig. 3
From: Myocardin regulates mitochondrial calcium homeostasis and prevents permeability transition
Myocardin opposes necrosis and mitochondrial dysfunction. a H9c2 cells were transfected with myocardin (Myocd) or an empty vector control, and treated with ionomycin (Iono, 2 µM, 18 h); DMSO was used as a control vehicle. Cells were stained with calcein-AM and Eth HD-1 to identify living (green) and necrotic (red) cells, respectively; b stained with calcein-AM and CoCl2. CMV-dsRed identifies positive cells. c Quantification of a by calculating the percentage of necrotic cells (Eth HD-1 Positive) and over 200 cells per condition and d by calculating the percentage of transfected cells with mitochondrial puncta, in 10 random fields. e H9c2 cardiac myocytes were pre-treated with CsA (1 µM, 2 h) followed by ionomycin treatment (Iono, 2 µM, 18 h). Cells were stained with calcein-AM and CoCl2 and quantified. (f) H9c2 cardiac myocytes transfected with Myocd or an empty vector control, treated with staurosporine (STS, 2uM, 18 h); cell viability was assessed by MTT analysis (n = 4). Data are expressed as mean ± SE. *p < 0.05 compared to control, **p < 0.05 compared to treatment, determined by 1-way ANOVA
