Fig. 6
From: Myocardin regulates mitochondrial calcium homeostasis and prevents permeability transition
Myocardin regulates mitochondrial permeability transition and calcium homeostasis through Nix. a H9c2 cells were transfected with sh-Myocd, sh-Nix, as indicated, a mito-targeted calcium biosensor and quantified (b). H9c2 cells were transfected with sh-Myocd, sh-Nix; as in (A) and stained with calcein-AM and CoCl2 and quantified. c H9c2 cells were transfected with Nix or empty vector control, treated with cyclosporine A (CsA, 1 µM, 18 h); stained with calcein-AM and CoCl2 and quantified. d, e H9c2 cells were transfected with Nix or empty vector control and (d) the SR-targeted calcium biosensor or (e) the mito-targeted calcium biosensor, and quantified. f H9c2 cells were transfected with sh-Nix or a scrambled control, treated with FSK-I (18 h), or DMSO as a control vehicle; stained with calcein-AM and CoCl2 and quantified. g H9c2 cells were transfected with sh-Myocd or scrambled control. Mitochondrial and ER fractions were immunoblotted as indicated. h H9c2 cells were tansfected with Nix, mitochondrial-targeted Nix (Nix-Mito), or SR-targeted Nix (Nix-SR), and the mito-targeted calcium biosensor, and quantified. i H9c2 cells were transfected with Nix, wild-type Bcl2, mitochondrial-targeted Bcl-2 (Bcl2-M), or SR-targeted Bcl-2 (Bcl2-SR), or empty vector control, stained with calcein-AM and CoCl2 j or transfected with mito-targeted calcium biosensor, and quantified
