Fig. 7
From: Myocardin regulates mitochondrial calcium homeostasis and prevents permeability transition
In vivo analysis of the myocardin-miR133a-Nix pathway. a–d Sprague Dawley rats were subjected to left coronary artery ligation, or sham operation as a control. Following 4-weeks of recovery, the viable infarct border-zone was harvested from the left ventricle. Extracts were analyzed by real-time PCR for Myocardin (a), or miR-133a (b) expression (n = 4). Protein extracts were immunoblotted for Nix (c) expression and subjected to densitometry (d, n = 3). (E-H) C57BL6 mice received weekly intraperitoneal injections of doxorubicin (Dox; 8 mg/kg), or vehicle control, for 4 weeks. Extracts were analyzed by real-time PCR for Myocardin (e), or miR-133a (f) expression (n = 4). Protein extracts were immunoblotted for Nix (g) expression (n = 4) and subjected to densitometry (h). Data are expressed as mean ± SE. *p < 0.05 compared to control, determined by Student's T-test
