Fig. 2
From: p73 coordinates with Δ133p53 to promote DNA double-strand break repair
p73 promotes homologous recombination (HR), non-homologous end joining (NHEJ) and single-strand annealing (SSA) repair pathways. a, b Effects of p73 on HR, NHEJ and SSA repair frequencies in H1299 cells (p53−/−, p73+/+). The corresponding plasmids were linearised using I-SceI. H1299 cells were transfected with constructs corresponding to each of the three repair assays and a non-specific siRNA control (siNS), two p73 siRNAs [siRNA1 (p73i-1) or siRNA2 (p73i-2)], a CMV-Δ133p53 plasmid, the CMV-Δ133p53 plasmid together with each of the two p73 siRNAs or the CMV-p73 or CMV-Δ133p53 plasmid together with CMV-p73 plasmids. Western blot of p73 and Δ133p53 expression in H1299 cells transfected with different reagents as indicated (a). The average repair frequencies were measured using a quantitative (q) PCR analysis of repaired assay constructs from three repeat experiments at 24 h post treatment (b). c, d Effects of p73 on HR, NHEJ and SSA repair frequencies in Saos-2 cells (lacking both endogenous p73 and p53 proteins). Western blot of p73 and Δ133p53 expression in Saos-2 cells transfected with different reagents as indicated (c). The average repair frequencies were analysed as described for H1299 cells (d). All statistically significant differences between treatments were assessed using the independent-samples T-test (*P < 0.05, **P < 0.01)
