Fig. 6
From: p73 coordinates with Δ133p53 to promote DNA double-strand break repair
Δ133p53 and p73 act synergistically to bind to the promoters of RAD51, RAD52 and LIG4. a New types of p53 REs relevant for Δ133p53 transcription activation in the promoters of RAD51, RAD52 and LIG4. The black and red arrows correspond to the orientations of the quarter sites of p53 (RE). R = A or G, W = A or T, Y = C or T. The numbers indicate the positions of p53 REs in the three gene promoters. The p53 REs are indicated by uppercase letters. Mismatch nucleotides in p53 REs are underlined. The p73 REs are in green letters. Mismatch nucleotides in p73 REs are labelled with red letters. b Chromatin immunoprecipitation (IP) of p53 and p73 REs in the RAD51, RAD52 and LIG4 promoters in HCT116 cells at 24 hpi. HCT116 cells were transfected with non-specific siRNA (siNS) or Δ133p53 interference (Δ133p53i), followed with 10 Gy of γ-ray irradiation. An N-terminal p73 antibody was used for protein–DNA complex IP. IgG was used as a non-specific binding control. Specific primer pairs were designed to amplify the corresponding REs. DNA was normalised to β-ACTIN (negative control primers). The results are presented as relative occupancies of the different REs. Statistics were obtained from three repeat experiments. c IP of p53 and p73 REs in the RAD51, RAD52 and LIG4 promoters in Saos-2 cells transfected with HA-p73, HA-Δ133p53 or co-transfected HA-p73 with Myc-Δ133p53. The transfected cells cells were sampled at 24 hpt. A HA antibody was used for protein–DNA complex IP. The ChIP assay was performed as described in b
