Fig. 1
Genetic deletion of TRIF significantly shortens the life span of SOD1G93A mice by accelerated disease progression. a Survival curves of SOD1G93A (black, n = 16), SOD1G93A/TRIF+/− (orange, n = 56), and SOD1G93A/TRIF−/− (light blue, n = 21) mice produced by a double mating of SOD1G93A and TRIF−/− mice. b Plotted mean survival time of each genotype showed that heterozygous and homozygous deletion of TRIF gene shortened survival times of SOD1G93A mice. Data are presented as mean ± SD. c Survival curve of SOD1G93A (black, n = 34), SOD1G93A/MyD88+/− (yellow, n = 62), and are SOD1G93A/MyD88−/− (green, n = 26) mice produced by a double mating of SOD1G93A and MyD88−/− mice. d Plotted mean survival times showed that the survival times of SOD1G93A mice were not affected by MyD88 gene deletion. Data are presented as mean ± SD. e, f Ages of disease onset (e) and disease end stage (f) for SOD1G93A/TRIF−/−/MyD88−/− (red, n = 5), SOD1G93A/TRIF−/− (blue, n = 7), SOD1G93A/MyD88−/− (green, n = 4), and littermates SOD1G93A mice (black, n = 6) produced by a triple mating of SOD1G93A, TRIF−/−, and MyD88−/− mice. g Mean duration of disease (days from onset to end stage) for SOD1G93A/TRIF−/−/MyD88−/− (red), SOD1G93A/TRIF−/− (blue), SOD1G93A/MyD88−/− (green), and littermate SOD1G93A mice (black). h Mean survival time of each genotype shown in f. Data are presented as mean ± SD. Statistical analyses of survival time (b, d, h) and disease duration (g) were performed with log-rank tests with Bonferroni corrections for multiple comparisons and one-way ANOVA followed by Tukey–Kramer multiple comparison post hoc tests, respectively. *p < 0.05, **p < 0.01, ****p < 0.0001
