Fig. 2

LincRNA-Cox2 knockdown upregulates autophagy by inhibiting Nlrp3 expression. a, b Q-PCR and Western blot detection of NLRP3 in control or lincRNA-Cox2 knockdown of BV2, BMDM and primary microglia treated with LPS for 4 h. c Q-PCR and Western blot detection of NLRP3 in control or lincRNA-Cox2 knockout (KO) of BV2. d–f Western blot of LC3-I and LC3-II in control or lincRNA-Cox2 knockdown of BV2, BMDM and primary microglia following treatment with Nlrp3 siRNA and 4 h LPS add 1 mM ATP for 30 min administration. g–i Ratio of LC3-II/LC3-I in three independent experiments as in (d–f). j–l Levels of IL-1β secreted in control or lincRNA-Cox2 knockdown of BV2, BMDM and primary microglia following treatment with Nlrp3 siRNA, 4 h LPS add 1 mM ATP for 30 min administration. GAPDH is shown as the loading control in all western blot figures (western blot repeated in three independent experiments). Columns are the mean values of triplicates; the error bar indicates the SEM. Asterisks indicate statistically significant difference from each other; **p < 0.01, *p < 0.05