Fig. 4
From: TSPYL2 is a novel regulator of SIRT1 and p300 activity in response to DNA damage
TSPYL2 regulates p300-SIRT1 association. a, b U2OS cells were transfected with MOCK or FLAG-TSPYL2 encoding vectors and treated or not with etoposide. TSPYL2 association with SIRT1 a and p300 b was assessed by proximity ligation assay. c western blot analyses of p300 (top panel) and SIRT1 (bottom panel) immunocomplexes from U2OS cells treated or not with etoposide. IP, immunoprecipitates; PC, negative control; Input, total lysate. In the charts, densitometric quantification of SIRT1 co-immunoprecipitated with p300 (top) and p300 co-immunoprecipitated with SIRT1 (bottom). Data from etoposide treated samples were normalized to those from untreated cells. Values are mean ± standard deviation (s.d.) of three independent experiments. d SIRT1 was immunoprecipitated from control or TSPYL2-depleted cells before and after etoposide treatment. p300 presence in the immunocomplexes was evaluated by western blot. IP, immunoprecipitates; PC, negative control; Input, total lysate. The fold induction of co-immunoprecipitated p300 relative to immunoprecipitated SIRT1 is reported in the chart. Data were normalized to that from untreated siLUC cells and values are mean ± s.d. from three independent experiments
