Fig. 2

SW620 cells undergo into EMT. a RT-qPCR analysis for HOTAIR and for the indicated epithelial (ECAD, HNF1A, HNF4A) and mesenchymal (VIM, FN1, Snail) markers on SW620 cells treated or not with TGFβ. The values are calculated by the ΔCt method and expressed as relative to the L32 RNA levels and shown as mean ± S.E.M. Statistically significant differences (*p < 0.05; **p < 0.01) are reported for four independent experiments. b Western blot analysis for the indicated markers on protein extracts from cells as in a. Protein amount was normalized by immunoblotting for GAPDH, as indicated. All the experiments were performed in triplicate and the WB images represented as one indicative experiment of three independent experiments