Fig. 3

HOTAIR has a functional role in EMT of colon cells. a RT-qPCR analysis for the indicated epithelial (ECAD, HNF1A, HNF4A) and mesenchymal (MMP2, VIM, FN1, Snail) markers in HOTAIR-silenced cells (siHotair) in comparison with siGFP cells (siCtr), as control. The values are calculated by the ΔCt method, expressed as fold of expression vs. the control (arbitrary value = 1) and shown as means ± S.E.M. Statistically significant differences (*p < 0.05; **p < 0.01; ***p < 0.001) are reported for five independent experiments. b Western blot analysis for the indicated markers on protein extracts from cells treated as in a. Protein amount was normalized by immunoblotting for GAPDH, as indicated. All the experiments have been performed in triplicate and WB images represent one indicative experiment of three independent ones