Fig. 4
From: The lncRNA HOTAIR transcription is controlled by HNF4α-induced chromatin topology modulation
Snail function in colon cells. a qPCR analysis of ChIP assays with anti-Snail antibody (IP Snail) and, as controls, normal rabbit IgG (IgG) on chromatin from SW480 and SW620 cells. Data show the direct recruitment of endogenous Snail on the correspondent consensus binding sites (E-boxes) on the human ECAD promoter. Rpl30 sequences are analyzed as negative control (Neg Ctr). Values derived from five independent experiments are reported as means ± S.E.M. and expressed as percentage of the Input chromatin (% Input). Statistically significant differences (*p < 0.05; **p < 0.01; n.s. no significant) are reported. b qPCR analysis of ChIP assays with anti-H3K27me3 antibody (IPH3K27me3) and, as controls, normal rabbit IgG (IgG) on chromatin from SW480 and SW620 cells. Data show the enrichment of H3K27 trimethylation on the Snail consensus binding sites (E-boxes) on the human ECAD promoter. Rpl30 sequences are analyzed as negative control (Neg Ctr). Values derived from five independent experiments are reported as means ± S.E.M. and expressed as percentage of the Input chromatin (% Input). Statistically significant differences (*p < 0.05; **p < 0.01; n.s. no significant) are reported
