Fig. 2
Leukocytosis in sick primary AML mice. a Blood smears typical of mice reconstituted with stem/progenitor cells of the following genotypes: WT/MLL-AF9 (mouse #2036), Mcl-1tg/MLL-AF9 (mouse #2039), BCL-2tg/MLL-AF9 (mouse #2041) and Bim-/-/MLL-AF9 (mouse #1250). Scale Bars: 20 μm, ×1000 magnification. A typical blast cell is indicated by the black arrowhead and a differentiated myeloid cell with a red arrowhead. (See Supplementary Figure S4 for bone marrow cytospins). b, c Quantification of blast cells (indicated by high nucleus to cytoplasm ratio, scored blinded) in (b) blood and (c) bone marrow of sick primary WT/MLL-AF9 (orange; blood n = 14, BM n = 9), Mcl-1tg/MLL-AF9 (pink; blood n = 18, BM n = 13), BCL-2tg/MLL-AF9 (blue; blood n = 16, BM n = 8) and Bim-/-/MLL-AF9 (black; blood n = 6, BM n = 5) mice. Each dot represents one mouse *p < 0.05, **p < 0.01, ***p < 0.001 calculated by Student’s T-test with Welch’s correction
