Fig. 1

MALAT1 is induced by UV irradiation and is overexpressed in cSCC cell lines and tumors. a Expression levels of MALAT1 were detected in cSCC cell lines and control HaCaT keratinocytes by qRT-PCR. b Expression levels of MALAT1 were detected in the normal tissues and cSCC tumors by qRT-PCR. c RNA ISH staining of MALAT1 on cSCC specimens. MALAT1 staining images representing normal tissue and low, medium, and high cSCC differentiation grades are shown. Positive MALAT1 signals are NBT/BCIP precipitates in a purple blue color and indicated by arrow heads. d Association of MALAT1 ISH-staining scores with grades of cSCC tumor differentiation (low, medium, or high). Case numbers are indicated below. Data are plotted as the means of the 95% confidence intervals. All statistical data represent the average of three independent experiments ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 100 µm