Fig. 2
The overexpression of LINC00336 promotes cell growth, colony formation, tumor formation, and inhibits ferroptosis. a,b A qRT-PCR analysis was conducted to detect the level of LINC00336 in A549 (a) and SPC-A-1 (b) cells stably overexpressing LINC00336. c,d MTT assay was used to assess cell viability in A549 (c) and SPC-A-1 (d) cells stably overexpressing LINC00336. e,f Colony-formation assay of A549 (e) and SPC-A-1 (f) cells stably overexpressing LINC00336. g–l Nude mouse cells after the injection of A549 and SPC-A-1 stably expressing the control vector or LINC00336 expression plasmids are shown. Tumor formation was monitored at the indicated times (i,j), images (g,h) and weights (k,l) were recorded (n = 6). m,n MTT assays were used to analyze the responses of A549 (m) and SPC-A-1 (n) cells stably overexpressing LINC00336 to ferrostatin (2.0 μM), erastin (10.0 μM), and RSL3 (2.0 μM). o–x Levels of total iron (o,p), ferrous iron (q,r), lipid ROS (s,t), mitochondrial superoxide (u,v) and mitochondrial membrane potential (w,x) were analyzed in A549 and SPC-A-1 cells stably overexpressing LINC00336. Data are shown as the mean ± SEM; n ≥ 3 independent experiments, two-tailed Student’s t-test: ns nonsignificant (p > 0.05), *P < 0.05, **P < 0.01, ***P < 0.001
