Fig. 3
From: Brf1 loss and not overexpression disrupts tissues homeostasis in the intestine, liver and pancreas
Brf1 is essential for adult mouse liver function. a Polysome profiles from liver samples of Brf1+/+ mice (Ah-Cre Brf1+/+) harvested at day 8 post induction and Brf1fl/fl (Ah-Cre Brf1−/−) mice harvested at days 4 (n = 2), 6 (n = 3) and 8 (n = 4) post induction as shown. Brf1+/+ mice are a combination of Ah-CRE Brf1+/+ and mice without Ah-Cre (n = 3). b H&E stained mouse liver samples. Brf1+/+ mice are a combination of AhCRE Brf1+/+ and AhCre negative mice, whilst Brf1fl/fl mice are AhCre Brf1fl/fl mice. Day post-induction with β-naphthoflavone is indicated above the panels. Insets for day 6 and day 8 samples are 400x magnifications to better show tissue morphology. Arrows in the inserts show immune cell infiltration and collapsing hepatocytes. c Graph depicting mouse liver weight as a fraction of total body weight. Genotypes and day post-induction are shown on the x-axis. Brf1+/+ mice are a combination of AhCRE Brf1+/+ and AhCre negative mice, whilst Brf1fl/fl mice are AhCre Brf1fl/fl mice. P-values were calculated using a Mann–Whitney test to compare +/+ and fl/fl data. Changes at day 2 are not significant. Between 4 and 10 mice were analysed per genotype per time point. d IHC for p53 or p21, as indicated, on liver samples from mice harvested at 3, 4, 6 and 8dpi. Day post-induction with β-naphthoflavone is indicated above the panels. Brf1+/+ mice are a combination of AhCRE Brf1+/+ and AhCre negative mice, whilst Brf1fl/fl mice are AhCre Brf1fl/fl mice
