Fig. 2
From: Liver stage malaria infection is controlled by host regulators of lipid peroxidation
SLC7a11 pathway is responsible for P53-mediated elimination of LS-infected hepatocytes. a Schematic of signaling induced by P53. b, c 1.5 × 105 Hepa1-6 cells were treated with 10 µM Nutlin-3. Cellular lysates were isolated 24 h after treatment and immunoblots against the indicated targets were performed. Signal was quantified using Image Studio software and normalized to βActin. d 1.5 × 105 Hepa1-6 cells were transduced with lentivirus expressing shRNAs against a scramble control, NOX1 or TFR1 and infected with 5 × 104 P. yoelii sporozoites. After 90 min post-infection, cells were treated with 10 µM Nutlin-3 or a DMSO control. Parasites were visualized 24 h post-infection by Hsp70 staining and quantified by microscopy. Each bar represents the mean of replicates. P-values were obtained using a Student’s t-test
