Fig. 1

Among TNBC/basal-like enriched-lncRNAs, 10 are enriched in tumorigenic Aldefluor^high cells and NRAD1 is also associated with poorer survival in basal-like breast cancers. a Aldefluor^high (ALDE+) and Aldefluor^low (ALDE−) cell populations isolated from PDX 7482 (top) and SUM149 (bottom). The inclusion of a DEAB-treated control confirms that Aldefluor^high cells were correctly identified. b Transcript levels of ALDH1As in Aldefluor^high versus Aldefluor^low PDX 7482 or SUM149 cells. c Aldefluor^high or Aldefluor^low sorted populations of TNBC PDX 7482 (top) are assessed for tumor growth potential in mice (n = 6 per group for SUM149, n = 3 per group for PDX 7482). Error bars represent standard error. d Transcript levels of lncRNAs in Aldefluor^high versus Aldefluor^low PDX7482 cells or SUM149 cells detected by QPCR. The data are normalized to reference genes PUM1 and ARF1 and the log2 fold change over the Aldefluor^low mRNA levels (log2 = 0). Error bars represent standard error. e A venn diagram shows the number of lncRNAs that were expressed at least 2-fold more (log2 ≥ 1) in Aldefluor^high versus Aldefluor^low PDX 7482 or SUM149 cells, and commonly in both TNBC models. f Regression-free survival in 360 basal-like breast cancer patients based on median expression of NRAD1 was analyzed using KMPlotter [38] (left), and in 106 basal-like breast cancer patients based on median expression of NRAD1 was analyzed using by extracting survival data from TCGA Cell 2015 dataset through cBioportal [39,40,41] (right). g Expression of NRAD1 in TNBC verus non-TNBC patient tumors, or basal-like versus non-basal-like tumors in the TCGA Cell 2015 dataset