Fig. 3
From: ATF3 promotes erastin-induced ferroptosis by suppressing system Xc–
ATF3-mediated suppression of system Xc− contributes to the promotion of ferroptosis. a, b HT1080 and RPE cells were treated with or without 10 μM of erastin for 1 h, and the amount of glutamate released into culture medium was measured. c, d Indicated cells treated with 10 μM of erastin for 5 h were lysed, and the intracellular GSH level was measured. e Viable HT1080 cells after treatments with erastin (1 μM) and/or 2-ME (20 μM) were counted. f Viable RPE cells treated with erastin (5 μM) and/or 2-ME (20 μM) were counted. g, h Cells were treated with erastin and/or NAC (3 mM), and subjected to MTT assays. The data are presented as mean ± SD. *p < 0.05; ***p < 0.001, Student’s t-test
