Fig. 6
From: LZTR1 facilitates polyubiquitination and degradation of RAS-GTPases
LZTR1 and RAS might contribute to autophagy. a Cells were seeded in 60 mm dishes and transfected with transfected with RAS and Myc-LZTR1 (5:1 µg ratio of RAS:LZTR1) for 24 h, followed by treatment with 50 µM chloroquine for 4 h. We evaluated LC3B-II levels by western blot. Band densities were analyzed using ImageJ software as in Fig. 1d. b Under the same conditions as in B, the cellular colocalization of Flag-RAS and LC3B in HEK293 cells. We then stained the cells with anti-LC3B (green), anti-Flag-tag (red), and NucBlue Stain (nuclei, blue). The yellow arrows indicate representative locations where RAS and LC3B overlap. We used MRAS-pcDNA, HRAS-pcDNA, NRAS-pcDNA and KRAS-pCAGGS expression plasmids
