Fig. 2
From: In vivo detection of programmed cell death during mouse heart development
PCD detection in transgenic CAG-sA5-YFP mouse embryos. Macroscopic pictures of WT and transgenic embryos at E9.5 (a–c) and E10.5 (d). Spotty bright green fluorescent signals (white arrows) mark PCD cells in the otic ring, the neural tube, the yolk sac, and the heart of E9.5 and E10.5 mouse embryos. e Close-up of a transgenic heart with sA5-YFP expression. f Macroscopic picture of a limb from a transgenic E13.5 embryo. Bright green fluorescence signals at the interdigital webbing (arrows) mark PCD cells. TL transmission light, sA5-YFP secreted Annexin V-yellow fluorescent protein, and Afl autofluorescence. Bars are 200 µm (a, b, f), 500 µm (c, d), and 20 µm (e)
