Fig. 5: Caspase-2-S384E expressing cells fail to cleave MDM2 and show increased polyploidy following AURKB inhibition.
From: Phosphorylation by Aurora B kinase regulates caspase-2 activity and function
GFP-expressing U2OS (sWT) and U2OS-CASP2−/− expressing GFP (sKO), GFP-caspase-2-C320G (sC320G) or GFP-caspase-2-S384E (sS384 #1 and #2) were treated with DMSO or 2 µM ZM447439 (ZM) for 48 h and subjected to immunoblot and DNA content analysis. a Representative immunoblots (from three independent experiments) of cell lysates from treated stable cell lines. Antibodies used for immunoblotting are as indicated. β-actin was used as loading control. b Representative flow cytometric profiles of the DNA content in cells following ZM treatment. The percentage represents polyploid cells (>4N), which was calculated using FACS Express 6 cell cycle analysis module. c Graph comparing percentage of cells with polyploid (>4N) DNA content following ZM treatment. Mean ± SEM; n = 3. *p < 0.05; **p < 0.01 compared to sWT + ZM.
