Fig. 7: Caspase-2-S384E expressing cells are resistant to aberrant mitosis-mediated cell death.
From: Phosphorylation by Aurora B kinase regulates caspase-2 activity and function
GFP-expressing U2OS (sWT) and U2OS-CASP2−/− expressing GFP (sKO), GFP-caspase-2-C320G (sC320G) or GFP-caspase-2-S384E (sS384 #1 and #2), were treated with DMSO or 100 nM BI2536 (BI) for 48 h and subjected to immunoblot and MTS assay. a Plot showing percentage of viable cells following BI treatment. Mean ± SEM; n = 3. *p < 0.05; **p < 0.01 compared to sWT. b Representative immunoblots (from three independent experiments) of cell lysates from treated stable cell lines. Antibodies used for immunoblotting are as indicated. β-actin was used as loading control.
