Table 2 The primers applied in current study.
From: Cell fate determined by the activation balance between PKR and SPHK1
Gene | Primer |
|---|---|
SPHK1a | |
Sense | CCCAAGCTTATGGATCCAGCGGGCGGCCC |
Antisense | CCGCTCGAGATGGATCCAGCGGGCGGCCC |
PKRa | |
Sense | CGCGGATCCATGGCTGGTGATCTTTCAG |
Antisense | CCCAAGCTTACATGTGTGTCGTTCATTTT |
SPHK1b | |
Sense | CCGGAATTCATGGATCCAGCGGGCGGCCC |
Antisense | CCGCTCGAGTCAATGGATCCAGCGGGCGGC |
PKRb | |
Sense | CGCGCATCCATGGCTGGTGATCTTTCAGCA |
Antisense | CCGCTCGAGCTAACATGTGTGTCGTTCATTTT |
D1c | |
Sense | CTAGTCTAGAATGGCTGGTGATCTTTCAGC |
Antisense | CCCAAGCTTCAAAGATCTTTTTGCCTTCCTTTG |
D2c | |
Sense | CTAGTCTAGAATGCGTGTTAAATATAATAACGA |
Antisense | CCCAAGCTTACATGTGTGTCGTTCATTTTTCT |
S1PR1d | |
Sense | GCCTACACAGCTAACCTGCTCTTG |
Antisense | TGGCGATGGCGAGGAGACTG |
S1PR2d | |
Sense | CCACCACCTCCTGCCACTCC |
Antisense | CACCGTGTTGCCCTCCAGAAAC |
S1PR3d | |
Sense | GATCCTCTACGCACGCATCTACTTC |
Antisense | ACACGCTCACCACAATCACCAC |
GAPDHd | |
Sense | AACGGATTTGGTCGTATTGG |
Antisense | GATTTTGGAGGGATCTCGC |
ABCC1d | |
Sense | CCGTGTTGGTCTCTGTGTTCCTG |
Antisense | AAGTCGGCGGCGTAATTCTTAGC |
SPNS2d | |
Sense | GTTACTGGCTGGCTGTGGCTTC |
Antisense | GCAACACTCGGACCTGGTTCTTG |
