Fig. 2: miR-142-5p can be transferred to human dermal lymphatic endothelial cells (HDLECs) via CSCC-secreted exosomes. | Cell Death & Differentiation

Fig. 2: miR-142-5p can be transferred to human dermal lymphatic endothelial cells (HDLECs) via CSCC-secreted exosomes.

From: Exosome-derived miR-142-5p remodels lymphatic vessels and induces IDO to promote immune privilege in the tumour microenvironment

Fig. 2: miR-142-5p can be transferred to human dermal lymphatic endothelial cells (HDLECs) via CSCC-secreted exosomes.

a and b The morphology and size of exosomes secreted by Siha and Caski cells transfected with lentivectors overexpressing miR-142-5p or NC were confirmed by transmission electron microscopy and Nanosight particle tracking analysis. Scale bar, 50 nm. c Characteristic marker expression (CD9, HSP70, and TSG101) of exosomes was confirmed by western blotting. d Confocal imaging showed the delivery of PKH67-labelled exosomes (green) to phalloidin-labelled HDLECs (red). Scale bar, 25 µm. e miR-142-5p levels in indicated exosomes were detected by RT-qPCR. f miR-142-5p levels in HDLECs pre-treated with PBS or indicated exosomes for 24 h were detected by RT-qPCR. S/miR-NC exo Siha/miR-NC exosomes, S/miR-142 exo Siha/miR-142-5p exosomes, C/miR-NC exo Caski/miR-NC exosomes, C/miR-142 exo Caski/miR-142-5p exosomes. Error bars represent the mean ± SD of three independent experiments. ***P < 0.001.

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