Fig. 3: SPAG5 depletion affects proliferation, clonogenicity, and migration of breast cancer cells. | Cell Death & Differentiation

Fig. 3: SPAG5 depletion affects proliferation, clonogenicity, and migration of breast cancer cells.

From: Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation

Fig. 3

A–D Cellular growth curves of MDA-MB-231 (A) and MDA-MB-468 (D) breast cancer cell lines determined by counting dye method after 24–48–72 h from siGFP and siSPAG5 tranfection. B–E Western blot with SPAG5 and p21 antibody of whole-cell lysates of MDA-MB-231 (B) breast cancer cell lines and cleaved-PARP and caspase 3 antibodies of whole-cell lysates of MDA-MB-468 (E) breast cancer cell lines harvested after 48 h from siGFP or siSPAG transfection. C–F Clonogenic assay of MDA-MB-231 (C) and MDA-MB-468 (F) breast cancer cell lines transfected with siGFP and siSPAG5 for 48 h before seeding at clonal density. G Kaplan–Meier plots show metastasis-free survival analysis of breast cancer patients with low and high expression of SPAG5. H–I Migration-assay histograms show the migrated cell count from MDA-MB-231 and MDA-MB-468 breast cancer cell lines determined by transwell assay after 72 h from siGFP or siSPAG5 transfection (H) and treated with 10ug/ml of mitomycin (I) (*P value <0.05; **P value <0.001).

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