Fig. 5: Foxo1 deficiency promotes β-catenin-mediated Gli1 activation and inhibits NEK7/NLRP3-driven inflammatory response in macrophages. | Cell Death & Differentiation

Fig. 5: Foxo1 deficiency promotes β-catenin-mediated Gli1 activation and inhibits NEK7/NLRP3-driven inflammatory response in macrophages.

From: Functional crosstalk between myeloid Foxo1–β-catenin axis and Hedgehog/Gli1 signaling in oxidative stress response

Fig. 5

BMMs were isolated from Foxo1FL/FL, Foxo1M-KO, β-cateninFL/FL, and β-cateninM-KO mice and incubated with LPS. A Quantitative RT-PCR-assisted analysis of Gli1 mRNA in LPS-stimulated BMMs (n = 3–4 samples/group). Western blots analysis and relative density ratio of B β-catenin and Gli1, C Foxo1, Gli1, and Snail, D NEK7, NLRP3, and cleaved caspase-1. Representative of three experiments. E ELISA analysis of supernatant IL-1β levels (n = 3–4 samples/group). All data represent the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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