Fig. 2: NBS1 deficiency leads to male infertility.

a Schematic illustrations of mating strategies for obtaining Nbs1 vKO mice. b Western blotting analyses of NBS1 in germ cells of Nbs1 vKO male mice at postnatal day 21 (P21). α-tubulin was used as loading control. c Cumulative numbers of pups per male obtained by mating control or Nbs1 vKO male mice with WT female mice. Three males of each genotype were used for mating. d H/E staining of paraffin sections of epididymis from control and Nbs1 vKO male mice (P42). Scale bar, 200 µm. e H/E staining of paraffin sections of ovaries from control and Nbs1 vKO female mice (P21). Scale bar, 200 µm. f Testes image of adult control and Nbs1 vKO male mice. g Testis to body weight ratios of control and Nbs1 vKO male mice (P7, P21, and P42). Error bars represent standard error of mean (SEM), n = 6. h H/E staining of paraffin sections of testes from control and Nbs1 vKO male mice (P21). Scale bar, 200 µm. i TUNEL staining of testis frozen sections from control and Nbs1 vKO mice (P21). The DNA was stained with DAPI. Scale bar, 20 µm. j Immunofluorescence staining analyses of PLZF in testis frozen sections from control and Nbs1 vKO mice (P21). Scale bar, 40 µm. The DNA was stained with DAPI.