Fig. 5: FLIP(L) cleavage promotes its interaction with SCFSkp2.
From: The SCFSkp2 ubiquitin ligase complex modulates TRAIL-R2-induced apoptosis by regulating FLIP(L)
a Co-IP analysis of the interaction of Cullin-1 with wild-type, non-cleavable (NC)-FLIP(L) and p43-FLIP(L). Interactions with endogenous Skp1, Skp2 and TRAF2 were also assessed. b Western blot assessment of degradation rates of wild-type-, NC- and p43-FLIP(L) using 100ug/mL cycloheximide (CHX). c Western blot of FLIP ubiquitination in HCT116 cells transfected for 48 h with siRNAs (20 nM) targeting Rbx1, Skp1 or Cullin-1. d Western blot analysis of FLIP ubiquitination in HCT116 cells transfected for 48 h with siRNAs (10 nM) targeting Skp2. g Impact of Cullin-1 and Skp2 over-expression on FLIP ubiquitination in the presence/absence of MLN4924 (300 nM, 3 h).
