Fig. 3: The molecular timer operates at all concentrations of pro-caspase 9 and APAF1.

Representative traces of IETDase activity after MOMP with abundant PC9 and APAF1 (a) or when one is limiting (b). Screening of the maximum IETDase activity (i), time to max IETDase activity (ii), half-time for IETDase decay (iii) and total IETDase activity (iv) after MOMP (c) or minimal MOMP (d). Half-times were calculated by fitting to one phase decay equation in Matlab. Total IETDase was calculated using the area under the curve.