Fig. 2: Attenuated UPR and apoptosis in cells deficient in STAU1.
From: Staufen 1 amplifies proapoptotic activation of the unfolded protein response
Western blots of cultured cortical neurons (a) or skin fibroblasts (b) from WT, Stau1+/−, or Stau1−/− mice incubated with thapsigargin (0.25 and 0.5 µM, 18 h). Graph represents quantification of target protein/actin from three independent experiments. c Cytotoxicity assessment by quantification of LDH release in WT or Stau1−/− mouse fibroblasts exposed to indicated doses of thapsigargin for 24 h. d Western blots of HEK293 cells transfected with siControl or siSTAU1 for 72 h and incubated with thapsigargin (0.5 and 1 µM) for 18 h. e mRNA levels of CHOP and ATF4 in HEK293 72 h post transfection with siControl or siSTAU1 and f after treatment with thapsigargin (0.5 µM) for the times indicated. Single asterisk (*), double asterisks (**), single dagger (†), or double daggers (††) denote significantly different from WT or siControl treated with the corresponding dose of thapsigargin. Data are mean ± SEM. * or †p < 0.05, ** or ††p < 0.01 by two-way ANOVA.
