Fig. 5: Protein level of Myst2 is decreased by proteasomal degradation during differentiation. | Cell Death & Differentiation

Fig. 5: Protein level of Myst2 is decreased by proteasomal degradation during differentiation.

From: BRPF3-HUWE1-mediated regulation of MYST2 is required for differentiation and cell-cycle progression in embryonic stem cells

Fig. 5

a Protein expression of Huwe1, Brpf3, and Myst2 during LIF-withdrawal-induced differentiation was analyzed by western blot. Alpha-tubulin was used as the loading control. b Protein expression of Myst2 after treatment with 10 μg/mL cycloheximide for 5 h during EB differentiation was investigated by western blot analysis. Alpha-tubulin was used as the loading control. c Protein level of Myst2 in Brpf3-overexpressing cells after treatment with 10 μM MG132 for 6 h during EB differentiation was analyzed by western blot. Alpha-tubulin was used as the loading control. d Protein level of Myst2-WT, K155R, and K576R during differentiation in Myst2-WT, K155R, and K576R stably expressed mESCs was analyzed by western blot. Differentiation was induced by withdrawal of LIF for 2days. Alpha-tubulin was used as loading control (upper panel). Quantification of results from western blot was normalized to that of alpha-tubulin. The expression level of control (Myst2-WT, LIF+) was set as 1. (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, compared with Myst2-WT (LIF-) (lower panel).

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