Fig. 4: Osteoclasts derived from p53−/− monocytes exhibit impaired functionality. | Cell Death & Differentiation

Fig. 4: Osteoclasts derived from p53−/− monocytes exhibit impaired functionality.

From: Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin

Fig. 4

a Representative images of osteoclast stained by T2RAP (red) after treatment for 7 days in specific medium plus 25 ng/ml M-CSF, 50 ng/ml RANKL, with or without OPG (50 ng/ml). Scale bar represents 50 µm. b, c Quantification of nuclei per osteoclast and TRAP + osteoclasts per field as shown in a. Quantification for Trap (d) and Itgb3 (e) mRNA expression of experiment shown in a. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, n.s. not significant. The experiments were repeated at least twice (biological replicates) with similar results.

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