Fig. 2: GLI1 forms complex with MVP and mTOR through the region of SUFU binding sites. | Cell Death & Differentiation

Fig. 2: GLI1 forms complex with MVP and mTOR through the region of SUFU binding sites.

From: Constitutive GLI1 expression in chondrosarcoma is regulated by major vault protein via mTOR/S6K1 signaling cascade

Fig. 2

A Silver staining results of IP with GLI1 antibody in SW1353 cell lysates. Normal IgG was used as the negative control. B SW1353 and HCS2/8 cells were lysed and subjected to IP-western blot assay to determine the interaction between endogenous GLI1 and MVP. C Immunofluorescence analysis of GLI1 and MVP distribution in CS cell lines. DAPI was used to stain nuclei. D The interaction between MVP and mTOR pathway components was examined using IP with MVP antibody. E HEK-293T cells were transiently transfected with empty vector or Flag-GLI1 together with GFP-MVP, cells were lysed and subjected to IP-western blot assay 48 h after transfection to examine the interaction of GLI1, MVP, mTOR, and p70S6K1. F HEK-293T cells were transiently transfected with MVP-GFP, GLI1-trunc1-Flag (SUFU binding sites), GLI1-trunc2-Flag (zinc-finger DNA-binding domain), and GLI1-trunc3-Flag (transcription activation domain). Cells were harvested 48 h after transfection followed by IP-western blot assay. G HEK-293T cells were transiently transfected with MVP-GFP, GLI1-trunc1-Flag, and SUFU-V5. Cells were harvested 48 h after transfection followed by IP-western blot assay.

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