Fig. 4: MVP promotes GLI1 expression by activating the mTOR/S6K1 signaling cascade. | Cell Death & Differentiation

Fig. 4: MVP promotes GLI1 expression by activating the mTOR/S6K1 signaling cascade.

From: Constitutive GLI1 expression in chondrosarcoma is regulated by major vault protein via mTOR/S6K1 signaling cascade

Fig. 4

A Western blot analysis of principle proteins involved in IHH, mTOR, and MAPK signaling pathway in control and CS cells with MVP knock-down. Densitometry analysis was performed to quantify the relative phosphorylation levels of p70S6K1, AKT, mTOR, and ERK based on the blots. B Western blot results showing the expression of indicated proteins in SW1353 cells over-expressing GLI1 and OUMS27 cells with GLI1 knockdown. C SW1353 and HCS2/8 were treated with dual PI3K/mTOR inhibitor PF-04691502(PF) for 24 h, cell lysates were subjected to western blot analysis with the indicated antibody. β-actin served as the loading control and the relative expression of GLI1 and phosphorylated p70S6K1 and mTOR are shown below the blots. D Real-time RT-PCR results showing the expression of genes related to the IHH pathway after treatment with PF-04691502 for 24 h.Error bars represent SD (n = 6). E Western blot analysis showing expression of proteins associated with IHH and mTOR pathway in HCS2/8 cells over-expressing MVP transfected with shp70S6K1 in the presence or absence of 10 ng/ml EGF. All data are presented as the mean ± SD (*p < 0.05, **p < 0.01,***p < 0.001, by Student’s t-test).

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