Fig. 5: MVP promotes proliferation and reduces cell apoptosis in CS cell lines. | Cell Death & Differentiation

Fig. 5: MVP promotes proliferation and reduces cell apoptosis in CS cell lines.

From: Constitutive GLI1 expression in chondrosarcoma is regulated by major vault protein via mTOR/S6K1 signaling cascade

Fig. 5

A Stable shMVP SW1353 cell clone was constructed. B Volcano plots showing the number of differentially expressed genes between shMVP transfected cells and the control group. C Heat map showing differentially expressed genes between shMVP transfected cells and control group, several down-regulated genes are listed in the right panel, including MVP and GLI1. Each sample was analyzed in triplicate. D KEGG analysis of the down-regulated genes after MVP knockdown. E RT-PCR on SW1353-shMVP cells was performed to validate the results from the RNA-seq. Error bars represent SD (n = 6). F Growth curves of CS cells over-expressing MVP or with MVP-knockdown. Error bars represent SD (n = 3). G Cell viability of clones with stable MVP knockdown was measured using CCK-8 assay. Non-target shRNA was used as a negative control. Error bars represent SD (n = 6). H Apoptotic cells were detected using the Annexin V/PI kit and results were analyzed by flow cytometry in siMVP and scramble NC group. I Western blot analysis of apoptosis-related proteins in shMVP transfected and control cells. All data are presented as mean ± SD (*p < 0.05, **p < 0.01,***p < 0.001, by Student’s t-test).

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