Fig. 1: RNAseq analysis identifies MECOM as uniquely expressed in dedifferentiated acinar cells.
From: MECOM permits pancreatic acinar cell dedifferentiation avoiding cell death under stress conditions
a Schematic overview of FACS-purification of human dedifferentiated acinar and duct cells, using acinar-specific UEA-1 lectin. b Immunofluorescent staining of FACS-purified dedifferentiated acinar (UEA-1+/CA19.9−) and duct (UEA-1−/CA19.9+) cells at day 4 with CD142 and CA19.9. DNA is labeled in blue. c qPCR validation of RNAseq data of purified human dedifferentiated acinar and duct cells: fold change expression (with HPRT as housekeeping gene) of purified dedifferentiated acinar cells (UEA-1+/CA19.9−) and duct cells (UEA-1−/CA19.9+) after day 4 of culture compared to the mixed exocrine fraction on day of isolation (day 0; DOI) (mean ± SD; N = 5; *p < 0.05; **p < 0.01; ***p < 0.001; one-way ANOVA with post-hoc Bonferroni correction). d Volcano plot of differentially expressed genes in human dedifferentiated acinar cells (green) and duct cells (gray). e KEGG pathways enriched (Adj. p-value < 0.01) in human dedifferentiated acinar cells (N = 5).
