Fig. 1: PRMT5 is upregulated in cervical cancer and promotes maintenance of stemness in cervical cancer cells.
From: Snail/PRMT5/NuRD complex contributes to DNA hypermethylation in cervical cancer by TET1 inhibition
A Immunohistochemical staining of PRMT5 in normal cervical tissues and cervical cancers (histological grades I, II, and III). Positively stained nuclei (in percentages) in grouped samples were analyzed by two-tailed unpaired t-test (*p < 0.05, **p < 0.01, ***p < 0.001). B Analysis of public datasets (GSE7803, GSE6791, and GSE67522) for the expression of PRMT5 by two-tailed unpaired t-test (*p < 0.05, **p < 0.01, ***p < 0.001). C Kaplan–Meier survival analysis of the relationship between survival time and PRMT5 in cervical cancer using the online tool. D HeLa cells were transfected with the shRNA or expression construct of PRMT5 for spheroid-forming assays. The images represent one field under microscopy in each group. E, F Expression of the indicated stemness markers was measured by real-time RT-PCR (E) or western blotting (F) in HeLa cells, with spheroid cells in (D).
