Fig. 5: Genome-wide transcription target analysis of the snail/PRMT5/NuRD(MTA1) complex.
From: Snail/PRMT5/NuRD complex contributes to DNA hypermethylation in cervical cancer by TET1 inhibition
A Genomic distribution of Snail, PRMT5, and MTA1 determined by ChIP-seq analysis. B Venn diagram of overlapping promoters bound by Snail, PRMT5, and MTA1 in HeLa cells. C Pathway analysis of the 170 overlapping target genes of PRMT5/Snail/MTA1 arranged into functional groups. D Visualized peaks at representative loci using an integrative genomics viewer. E Snail-, PRMT5-, and MTA1-bound motifs were analyzed using MEME suite. F, G Verification of ChIP-seq results by qChIP analysis of the indicated genes in HeLa cells. Results are represented as the fold-change compared to the control with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a negative control. Error bars represent the mean ± SD for three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, and two-tailed unpaired t-test).
