Fig. 1: ATP5F1C protein expression is increased in lymph nodal (LN) metastases: immunostaining with monoclonal antibodies directed against ATP5F1C.

Matched formalin-fixed paraffin-embedded (FFPE) tissue sections of the primary tumor (Left) and lymph nodal metastases (LN; Right), from the same patients, were subjected to immunohistochemical staining with mono-specific antibodies that recognize ATP5F1C. Note that ATP5F1C expression is increased in lymph node metastases, as compared to the matched primary tumor samples. Three representative examples are shown, from two luminal ER(+) breast cancer (BC) patients and one triple-negative breast cancer (TNBC) patient. Images were acquired with a 20× objective. Scale bar = 100 µm. Please also see Supplemental Fig. S6.