Fig. 6: MLKL facilitates G-CSF release by controlling membrane permeabilization.
From: MLKL promotes cellular differentiation in myeloid leukemia by facilitating the release of G-CSF
A Release of G-CSF in WT and Mlkl−/− BM cells measured in FLT3-ITD+ BM cells treated with or without PEG 8000 after 24 h (t-test, mean with SD). B Shown is the rate of cell death (PI+) of FLT3-ITD WT or Mlkl−/− BM cells after 24 h of PEG8000 treatment (t-test, mean with SD). C Supernatant G-CSF levels of WT or Mlkl−/− FLT3-ITD BM cells treated with PEG2000, PEG3000, PEG4000, PEG5000, PEG6000, and PEG8000 for 24 h (P-value between PEG4000 and higher by Student’s t-test). D Supernatant G-CSF levels of WT or Mlkl−/− BM-derived macrophages (BMDMs) treated with LPS and increasing sizes of PEG for 24 h. t-test, mean with SD. *P < 0.05, **P < 0.005, ***P < 0.0005.
