Fig. 7: MLKL associates with early endosomal marks during endosomal trafficking.
From: MLKL promotes cellular differentiation in myeloid leukemia by facilitating the release of G-CSF
A Immunofluorescence images, the 5-FU challenged bone marrow cells treated with 20 ng/ml LPS for 48 h (WT or Mlkl−/−) stained as follows; gray, G-CSF; magenta, EEA1; yellow, phalloidin (which stains the plasma membrane-associated F-actin), negative and second antibody only stain FACS in Fig. S7A and B, scale bar = 100 µm. B Quantification of the immunofluorescence images, the co-localization of G-CSF and EEA1, Pearson’s coefficient (t-test, mean with SD). C Shown is the level of G-CSF in the supernatant of WT or Mlkl−/− BM cells treated with LPS measured at serial time points (t-test, mean with SD). D Shown is the level of intracellular G-CSF in WT or Mlkl−/− BM cells treated with LPS measured at serial time points (normalized to the median of the baseline WT group, mean with SD). *P < 0.05, **P < 0.005, ***P < 0.0005.
