Fig. 3: Loss of tumorigenic activity by YB-1 inactivation is associated with a suppression of stress response proteins. | Cell Death & Differentiation

Fig. 3: Loss of tumorigenic activity by YB-1 inactivation is associated with a suppression of stress response proteins.

From: De novo and cell line models of human mammary cell transformation reveal an essential role for Yb-1 in multiple stages of human breast cancer

Fig. 3

A Cell cycle analysis of shYB-1- or shScr-transduced MDA-MB-231 cells. B Western blots showing NRF2, HIF1α, G3BP1, CAIX and YB-1 levels (relative to GRB2) of MDA-MB-231 cells transduced with shYB-1 or shScr vectors grown in ultra-low attachment plates for 24 h. Individual data are from independent experiments (n = 3). C Western blots showing HIF1α and YB-1 levels (relative to GAPDH) in shYB-1 and shScr-transduced MDA-MB-231 cells maintained in vitro for up to 24 h in 1% O2. D, E Representative images of CAIX (D)-, G3BP (E)- stained sections of tumours produced from shYB-1- or shScr-transduced MDA-MB-231 cells. Scale bar, 100 μm. Bar graph shows measured levels of CAIX or G3BP (staining intensity). F Dot plots of tumour sizes obtained in mice injected SQ with T47D cells transduced with GFP-control and KRASG12D and assessed 30 days post-transplant. Data are from individual mice (GFP, n = 3; KRASG12D n = 9). G Representative views of YB-1 levels in the IHC-stained in vivo progeny of GFP-control and KRASG12D-transduced T47D cells. Bar graph shows quantification of YB-1 expression. Data are from individual mice (GFP, n = 3; KRASG12D, n = 9).

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