Fig. 2: Local and systemic pSTAT3-dependent inflammation in vFLIP mice.

A Representative H&E-stained microscopy images of lung of WT (upper panel) or vFLIP chimera mice. Scale bar, 200 μm. B Representative IF staining of lung-infiltrating neutrophils (NE⁺ cells) and mononuclear phagocytes (CD68⁺ cells) in WT or vFLIP mice. Cells were stained for DAPI (blue), neutrophil elastase (NE) (red, middle panel), and CD68 (green, bottom panel). Scale bar, 200 μm. C Representative IF staining of lung-infiltrating neutrophils and mononuclear phagocytes in WT or vFLIP mice. Scale bar, 20 μm. Cells were stained for DAPI (blue), NE (red, middle panel) or CD68 (green, bottom panel), and pSTAT3 Tyr705 (gray). D P-selectin (CD62P) presence in lung of WT (upper panel) or vFLIP mice by H&E staining. Scale bar, 100 μm. E Representative confocal analysis of NET in the lung of WT (left panel; 50 μm) or vFLIP mice (200 μm). Cells were stained for DAPI (blue), NE (red), and H3cit (green). F Dot plots of IL-6 and TNF-α in lung-infiltrating mononuclear phagocytes (CD45.2⁺Ly6C⁺ cells) and neutrophils (CD45.2⁺Ly6G⁺ cells) isolated from vFLIP mice (n = 17). G Representative IF staining of spleen-infiltrating neutrophils (NE⁺ cells) and mononuclear phagocytes (F4/80⁺ cells) in WT or vFLIP mice. Cells were stained for DAPI (blue), NE (red, middle panel), and F4/80 (green, bottom panel). Scale bar, 200 μm. H Representative IF staining of spleen-infiltrating neutrophils and mononuclear phagocytes in WT or vFLIP mice. Cells were stained for DAPI (blue), NE (red, middle panel), or F4/80 (green, bottom panel), and pSTAT3 Tyr705 (gray). I Dot plots of IL-6 and TNF-α in mononuclear (Ly6C⁺ cells) and polymorphonuclear (Ly6G⁺ cells) myeloid cells in spleen of WT (n = 8) or vFLIP mice (n = 9). Data are reported as mean ± S.E.M. *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 by Mann–Whitney test.