Fig. 3: Lung immune landscape in vFLIP mice affected by cytokine release syndrome.
From: Fatal cytokine release syndrome by an aberrant FLIP/STAT3 axis
A tSNE representation of scRNA-seq from 2 WT and 2 vFLIP mice samples (WT: 9174; vFLIP: 9847) colored according to cell type. Stacked bar plots representing cell-type proportions across WT and vFLIP conditions. B Bar plot representing the upregulated (NES > 0, adjusted p-value <0.05) hallmark gene sets in the analysis of vFLIP vs WT cells obtained through GSEA analysis. C Violin plots showing the expression of key genes that drive the upregulation of inflammatory response, TNF-α signaling via NF-κB and JAK-STAT3 signaling pathway in the lung of vFLIP mice. The asterisks denote statistically significant upregulation in the comparison between vFLIP and WT conditions (*p < 0.05, **p < 0.01, ***p < 0.001). D Bar plot representing the upregulated (NES > 0, adjusted p-value <0.05) hallmark gene sets in bulk RNA-seq data obtained through GSEA analysis of ACE2-transgenic mice infected with Sars-CoV-2 [88] comparing day 7 post infection (dpi) with mock-infected (0 dpi). Red bars refer to the gene sets enriched in both vFLIP and ACE2-transgenic mice.
