Fig. 3: Synergistic effect of select compounds with remdesivir using HCoV-OC43 viral infection assay.
From: The NSP14/NSP10 RNA repair complex as a Pan-coronavirus therapeutic target
a Schematics showing the viral infection assay workflow. Drug treated cells were infected with HCoV-OC43 or SARS-CoV-2, fixed, stained, and imaged at indicated times post infection. Cytotoxicity was measured in similarly drug-treated uninfected cells using either the CellTiter-Glo or the alamarBlue assay. b Representative graphs are shown of the antiviral activity (full symbols) and cytotoxicity (empty symbols) of compounds #79, #96 and #102 in HCM3 cells infected with HCoV-OC43. Compounds were applied at the following concentrations: #79: 60 μM, #96: 15 μM, #102: 25 μM. Remdesivir concentrations are indicated on the X-axis in μM. Error bars represents SEM. IF images of representative wells show anti-N staining (red) and DAPI signal (blue) at indicated drug concentrations. Graph shows the EC50 values from three independent experiments using technical triplicates. Error bars represents SEM.
