Fig. 3: Kdm6b regulates TCRαβ⁺CD8αα⁺ IEL development in a cell-intrinsic manner.

a 1:1 ratio of bone marrow cells (5 × 10⁶) from Kdm6b-cKO (CD45.2) and Kdm6b-WT (CD45.1) mice were transplanted into irradiated (at 600 rads) RAG2^−/− mice (on a CD45.1 background). Recipients were analyzed 8 weeks later. b Flow cytometry analysis for the expression of TCRβ and TCRγδ in CD45.1⁺ and CD45.2⁺ IELs from the small intestines of recipient mice. c Frequencies of TCRαβ⁺ and TCRγδ⁺ IELs from the experiment in (b) (n = 4). d Small intestinal IELs from recipient mice were stained with anti-CD45, TCRβ, TCRγδ, CD4, CD8α, and CD8β. The cells were evaluated by flow cytometry. e Percentages of indicated subsets from the experiment in (d) (n = 4). f IELPs in thymocytes of recipients were analyzed by flow cytometry. g Frequencies of CD45.1⁺ and CD45.2⁺ IELPs from the experiment in (f) (n = 4).